Pharmacological assay showed that abi5-1 mutant was insensitive to TOR inhibitor AZD8055, whereas AtABI5 overexpression lines were hypersensitive to AZD8055 in Arabidopsis. Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell.

ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

Each graph 2016-12-20 Overexpression of ABI5 in plants to prevent precocious seed germination and to confer resistance to drought and high salt Mar 21, 2001 - The Rockefeller University The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants. ABI5-mediated transactivation was inhibited by overexpression ofabi1-1, the dominant-negative allele of the protein phosphatase ABI1, and by 1-butanol, a competitive inhibitor of … 2008-03-10 SnRK1α1 Antagonizes Cell Death Induced by Transient Overexpression of Arabidopsis thaliana ABI5 Binding Protein 2 (AFP2) September 2020 Frontiers in Plant Science 11 Srinivas S. L. Gampala, Ruth R. Finkelstein, Samuel S. M. Sun, Christopher D. Rock 2019-08-09 Our data show that loss of FyPP function is sufficient to trigger ABI5-dependent inhibition of seed germination (Figure 2B), which is very similar to the previous report that overexpression of a SnRK2 kinase (PKABA1) in the ABI5-OE background is sufficient to activate ABI5 protein and suppress seed germination (Piskurewicz et al., 2008). 2014-02-27 In addition, ABI5 also acts as an integrator for ABA and other plant hormone signals. Recent studies have shown that ABI5 is involved in photosynthesis by regulating chlorophyll metabolism [15,34,35]. To further analyze the function of ABI5 in chlorophyll metabolism, we treated the abi5-1 mutant and Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Author: Bi, Chao, Ma, Yu, Wu, Zhen, Yu, Yong-Tao, Liang, Shan, Lu, Kai, Wang, Xiao-Fang Source: Plant molecular biology 2017 v.94 no.1-2 pp. 197-213 ISSN: 0167-4412 Subject: Overexpression of FOF2 results in delayed transitions to flowering under both LD and SD conditions.FOF2 expression is induced by ABA during seed germination where it acts through ABI3 and ABI5 … The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants.

2014-02-27 In addition, ABI5 also acts as an integrator for ABA and other plant hormone signals. Recent studies have shown that ABI5 is involved in photosynthesis by regulating chlorophyll metabolism [15,34,35]. To further analyze the function of ABI5 in chlorophyll metabolism, we treated the abi5-1 mutant and Arabidopsis ABI5 plays a role in regulating ROS homeostasis by activating CATALASE 1 transcription in seed germination Author: Bi, Chao, Ma, Yu, Wu, Zhen, Yu, Yong-Tao, Liang, Shan, Lu, Kai, Wang, Xiao-Fang Source: Plant molecular biology 2017 v.94 no.1-2 pp. 197-213 ISSN: 0167-4412 Subject: Overexpression of FOF2 results in delayed transitions to flowering under both LD and SD conditions.FOF2 expression is induced by ABA during seed germination where it acts through ABI3 and ABI5 … The bZIP transcription factor ABI5 is shown to confer an enhanced response to exogenous abscisic acid during germination, seedling establishment and subsequent vegetative growth of plants.

These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5.

Overexpression of ABI5 reduces, whereas loss of function in the abi5 mutant increases, Cd accumulation in Arabidopsis, indicating the involvement of ABI5 in modulating Cd uptake. To identify transcriptional regulators involved in ABI5-mediated Cd accumulation, we searched for ABI5-interacting proteins and identified the Cd-induced R2R3-MYB TF MYB49 .

If ABI5 is a direct target of HY5, one would expect that overexpression of ABI5 may be able to rescue some of the hy5 mutant phenotypes such as its reduced sensitivity to ABA. The ABI5 overexpression lines were generated in both Col-0 and hy5 background 2020-04-11 · Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development. It has been demonstrated that activity of ABI5 is transcriptionally and post‐translationally regulated. 2019-06-01 · However, the overexpression of ABI5 in the bes1-D background could not rescue the early-flowering phenotype .

2020-03-02 · Consistently, PIFs and the G-box motifs in the ABI5 promoter determine ABI5 expression in darkness, and overexpression of ABI5 could rescue the ABA-insensitive phenotypes of pifq mutants in the dark. Moreover, we discovered that PIFs can physically interact with the ABA receptors PYL8 and PYL9, and that this interaction is not regulated by ABA.

Biochemical interaction assays demonstrated that ABI5 physically interacted with the RIBOSOMAL S6 KINASE2 (S6K2) protein in plant cell. It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

Overexpression of ABI3 and ABI5 simultaneously suppressed the ABA-insensitive phenotypes of the coi1-2 mutant and JAZ-accumulating (JAZ-ΔJas) plants. Together, our results reveal a previously uncharacterized signaling module in which JAZ repressors of the JA pathway regulate the ABA-responsive ABI3 and ABI5 transcription factors to integrate JA and ABA signals during seed germination and post-germinative growth.
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Consistent with this, seed size was also increased in abi5. We further show that ABI5 directly binds to two discrete regions in the SHB1 promoter.

ABI5 was previously reported as the target protein of AFP2 during seed germination (Lopez-Molina et al., 2003), and overexpression of ABI5 activates the flowering negative factor FLC at the transcriptional level to repress flowering (Wang et al., 2013b). Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions. Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days.
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ABI5 in the hy5 mutant background restored its ABA sensitivity and overexpression of ABI5 in the wild-type background-enhanced light response. These results indicate that HY5 inte-gratesbothABAandlightsignaltransductionpathwayspartially through direct activation of ABI5. Results HY5 Mediates ABA Response in Roots and Is Required for Osmotic

It has been known that ABA INSENSITIVE 5 (ABI5) plays a vital role in regulating seed germination. In the present experiment, we showed that 3-amino-1,2,4-triazole (3-AT) inhibits seed germination of the loss-of-function mutant abi5-1, but promotes seed germination of the ABI5-overexpression transgenic line, and that ABI5 affects reactive oxygen species (ROS) homeostasis. ABI5 overexpression rescued the growth arrest phenotype at low CO 2. Taken together, our results demonstrate that PPC2 and ABI5 are key regulators of plant acclimation to low CO 2, and positively contribute to carbon fixation and metabolism in C 3 plants.

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ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP transcription factors of the AREB/ABF family, which promote ABA responses. AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress.

This protects plants from drought. (a) ABI5‐HA fusion protein was detected by the anti‐HA antibody in ABI5 overexpression transgenic plants. (b) Analysis of the expression level of AtIPT8 by semi‐quantitative RT‐PCR. Seedlings were induced with 10 μ m estradiol for 24 h. The expression level of ACTIN2 represents the loading control.